課程資訊
課程名稱
 細胞再程序化專論
Special Topic in Reprogramming 
開課學期
 101-2 
授課對象
 生物資源暨農學院  生物科技研究所  
授課教師
 林劭品 
課號
 Biot5002 
課程識別碼
 642EU0040 
班次
  
學分
 2 
全/半年
 半年 
必/選修
 選修 
上課時間
 星期三5,6(12:20~14:10) 
上課地點
 生技所415 
備註
 本課程以英語授課。
限學士班三年級以上
總人數上限：15人 
Ceiba 課程網頁
 http://ceiba.ntu.edu.tw/1012Reprogramming 
課程簡介影片
 
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課程概述

Depends on the background of the students, 1/3 to 1/2 of the lectures will be given by the instructor for introducing basic concepts in all aspects of cellular reprogramming, including somatic cell nuclear transfer, stem cell differentiation and the application in regenerative medicine. For the rest of the classes, students will be assigned the most updated original research papers as well as the milestone publications. They will be randomly divided into 2 groups for each assignment. One group will be presenting the articles and students in the other group are expected to challenge the papers from the experimental design, the methodology to the interpretation of the data. Students in the 1st group should defend for the papers as if it’s their manuscript. Students from the 2nd group should also provide constructive advice as how to improve the manuscript so that it can be published in a higher impact journal. 

課程目標
 On top of learning the basic concepts of epigenetics and reprogramming, students are expected to develop the ability to critically evaluate and interpret published research articles. In addition, they will learn the art of reviewing as well as defending a manuscript for publication. 
課程要求
 Preferably having backgrounds in one of the following subjects: Biochemistry, Cell Biology, Genetics, Molecular Biology or Epigenetics 
預期每週課後學習時數
  
Office Hours
  
指定閱讀
  
參考書目
 Updated journal articles and reviews; “Epigenetics: Concepts, Theories, Paradigms and Mechanisms” and “Stem cell and Regenerative Medicine” CD-Roms (A Henry StewartTalks Series in the Life Sciences) 
評量方式
(僅供參考)
  
No.
項目
百分比
說明
1. 
 Participation during the course  
10% 
  
2. 
 Open book midterm exam  
30% 
  
3. 
 Oral presentations 
40% 
 "Authors" vs. "Reviewers" role playing 
4. 
 Final report 
30% 
 With the format of "mini-review" "preview"; "News and Views"or "Reviewers' comments 
 
課程進度
週次
日期
單元主題
第1週
 2/20  Introduction of Epigenetics, programming and reprogramming 
第2週
 2/27  Extended discussion in Epigenetics, programming and reprogramming 
第3週
 3/06  Transgenerational epigenetic reprogramming (I): Focusing on retrotransposon silencing 
第4週
 3/13  Transgenerational epigenetic reprogramming (II): Focusing on genomic imprinting
演講通知：3/19(二)在中研院早上11點，演講者為 Dr. Qing Nie演講題目：Stochastic Dynamics in cell Signaling and Developmental Patterning。請大家踴躍到場聆聽 
第5週
 3/20  3/20 邀請中研院陳俊安老師演講。
演講通知：3/26(二)在中研院分子生物研究所早上11點，演講題目：Molecular Mechanisms and Deep Sequencing-based Discovery of small Noncoding RNAs 
第6週
 3/27  Guided discussion I: Transgenerational reprogramming in germ cells and preimplantation embryos;
(Induced pluripotent stem cells and Trans-differentiation) 
第7週
 4/03  Somatic Cell Nuclear Transfer (Animal Cloning): An extreme example of artificial reprogramming 
第8週
 4/10  Small non-coding RNAs and functional genomics 
第9週
 4/17  Experimental reprogramming II 
第10週
 4/24  Commonly used techniques in Epigenetic related research 
第11週
 5/01  Open Book 期中考 
第12週
 5/08  Review of the midterm question set (Prof. SS Lin: The application of next generation sequencing in the understanding of RNA biology)
 
第13週
 5/15  The roles of functional non-coding RNAs: Extended reading
Epigenetic regulators and Reprogramming: Extended reading 
第14週
 5/22  Selection of final report topics The interactions among the 3 levels of epigenetic regulatory machineries 
第15週
 5/29  Author and Reviewer Role playing Part I
(Guided discussion)Epigenetics, programming,reprogramming and their implications in diseases and regeneration medicine 
第16週
 6/05  Authors and Reviewers Role playing Part II:LincRNA and epigenomic landscape shaping;
An example of physiological reprogramming: 蠑螈 (Salamandra salamandra) by Prof. Ben Lee 
第17週
 6/12  端午節 
第18週
 6/19  Term paper due: Please come to the classroom and finish the report before 3 pm incase you have not submitted it yet.